產(chǎn)品詳情介紹
PerfectStart? II Probe qPCR SuperMix利用在PCR體系中加入熒光探針 (TaqMan或Molecular Beacon等)，擴(kuò)增過程中其 熒光量與擴(kuò)增產(chǎn)物量成正比，通過熒光量的檢測(cè)測(cè)定樣本核酸量。本產(chǎn)品含PerfectStartTM Taq熱啟動(dòng)酶 (利用3種單克 隆抗體與Taq DNA Polymerase高效結(jié)合，有效地封閉了DNA聚合酶活性，阻止了低溫下的非特異性擴(kuò)增)、特殊優(yōu)化的qPCR反應(yīng)緩沖液、dNTPs、PCR增強(qiáng)劑、穩(wěn)定劑。本產(chǎn)品濃度為2×，使用時(shí)只需加入模板、引物、探針、Passive Reference Dye (根據(jù)qPCR儀選擇) 和水，使其工作濃度為1×，即可進(jìn)行反應(yīng)。
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