產(chǎn)品詳情介紹
該載體通過自殺基因表達(dá)與否篩選陽性重組子。當(dāng)載體與片段連接成功時(shí)，自殺基因無法正確表達(dá)，包含重組子的細(xì)胞可以正常生長(zhǎng)；當(dāng)載體與片段連接不成功時(shí)，自殺基因正確表達(dá)，包含載體的細(xì)胞無法生長(zhǎng)，即“Zero”背景。適用于TA克隆。
產(chǎn)品組成
References
1 Chen W, Chen L, Zhang X, et al. Convergent selection of a WD40 protein that enhances grain yield in maize and rice[J]. Science, 2022.(IF 63.71)
2 Zhao Z, Zhang Z, Li J, et al. Sustained TNF-α stimulation leads to transcriptional memory that greatly enhances signal sensitivity and robustness[J]. Elife, 2020.(IF 7.08)
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