產(chǎn)品詳情介紹
本產(chǎn)品通過(guò)在重疊區(qū)域引入突變位點(diǎn)的方法，利用TransStart? FastPfu Fly PCR SuperMix 擴(kuò)增，制備包含突變的PCR片段；利用特殊的重組酶和同源重組原理，將不多于6個(gè)片段無(wú)縫拼接，從而實(shí)現(xiàn)多點(diǎn)突變。
1 Li L, Fang C, Zhuang N, et al. Structural basis for transcription initiation by bacterial ECF σ factors[J]. Nature communications, 2019.(IF 11.90)
2 Deng L, Ren R, Liu Z, et al. Stabilizing heterochromatin by DGCR8 alleviates senescence and osteoarthritis[J]. Nature communications, 2019.(IF 10.70)
3 Wang Z, Wang S, Xu Z, et al. Highly promiscuous flavonoid 3-O-glycosyltransferase from Scutellaria baicalensis[J]. Organic letters, 2019.(IF 6.55)
4 Zhang L, Jiang D, Li Y, et al. Characterization of a novel type III alcohol dehydrogenase from Thermococcus barophilus Ch5[J]. International Journal of Biological Macromolecules, 2021.(IF 6.95)
5 Zhang L, Wang L, Wu L, et al. Biochemical characterization and mutational studies of a thermostable endonuclease III from Sulfolobus islandicus REY15A[J]. International Journal of Biological Macromolecules, 2021.(IF 6.95)
